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园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 507-508.

• 研究简报 • 上一篇    下一篇

中国茶组植物种质资源遗传多样性的RAPD分析

黎星辉1,2;章传政1; 刘春林2;施兆鹏2;罗军武2;陈 暄1   

  1. (1南京农业大学园艺学院, 南京210095; 2 茶学教育部重点实验室, 长沙410128)
  • 收稿日期:2006-08-17 修回日期:2007-02-16 出版日期:2007-04-25 发布日期:2007-04-25

RAPD Analysis of the Genetic Diversity in Chinese Tea Germplasm

LI Xing-hui1,2,ZHANG Chuan-zheng1,L IU Chun-lin2,SHI Zhao-peng2,LUO Jun-wu2, and CHEN Xuan1   

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2Key Laboratory ofM inistry of Education for Tea Science, Changsha 410128, China)
  • Received:2006-08-17 Revised:2007-02-16 Online:2007-04-25 Published:2007-04-25

摘要: 采用RAPD技术分析了中国茶组植物23个种质资源的遗传多样性。筛选的11个随机引物扩
增出135条RAPD谱带, 其中有多态带123条, 平均多态性为91.11%。聚类分析表明, 广南茶与大苞茶之间的遗传距离最小(0.1378) , 汝城白毛茶与厚轴茶之间的遗传距离最大(0.8995) , 应用UPGMA聚类分析法构建了DNA分子系统树图, 并据此讨论了茶组植物的分类。

关键词: 茶, RAPD, 遗传多样性

Abstract: The genetic diversity of the tea germplasm (Camellia Section Thea) in China was studied with RAPD method. 135 RAPD bands including 123 polymorphic bandswere scored in the 23 tea germplasm tested with 11 p rimers selected. The average of polymorphism was 91.11%. The results of cluster analysis indicated that the minimum genetic distance (0.1378) was between C.kwangnanica and C. grandibracteata, and the maximum genetic distance ( 018995 ) between C. pubescens and C. crassicolumna. A dendrogram by unweighted pair group method with arithmetic mean algorithm (UPGMA) analysis was obtained. Taxonomy for Camellia Section Thea was discussed by using the dendrogram.

Key words: Tea, Camellia Section Thea, RADP, Genetic diversity

中图分类号: