关键词: 番茄, 抗病基因, Tm-2²等位基因, 氨基酸序列

Abstract:

Alleles of Tm-2² from 4 different cultivars were cloned. Sequence analysis of them showed that: 1) 'Salong', 'Yukang-2' and '97-4' had 100% identity to reported sequences from 'GCR267'(Tm-2²),'GCR236'(Tm-2)and'GCR26'(tm-2), respectively; Cultivar 'yinghong1' had 98% identity to 'GCR26'(tm-2);2) in LRR domain of the encoded proteins, the resistant alleles Tm-22 and tm-2have two amino acid at sites of 624 and 704 different from that of the susceptible alleles tm-2, lptm-2, in which proline (P) changed to leucine (L) and methionine (M) to isoleucine (I); 3) in LRR domain of the encoded proteins between the sites of 601 and 790, 23 amino acid differences were found among Tm-2², Tm-2, lptm-2 and tm-2; 4) tm-2 of Yinghong1 (Lycopersicon esculentum.var.cerasiforme) might be an orthologous gene evolved from Lycopersicon esculentum cultivars.

Key words: Tomato, Resistant gene, Tm-2²alleles, Amino acid sequence