农杆菌介导菜豆铁结合蛋白基因(PvFer)转化番茄的研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 489-492.

农杆菌介导菜豆铁结合蛋白基因(PvFer)转化番茄的研究

叶霞^1,2;黄晓德¹;姚泉洪³; 章镇^1*

（¹南京农业大学园艺学院，江苏南京 210095；²河南黄河园林绿化工程有限公司，河南郑州450003；3上海市农业遗传重点试验室，上海 201106）

收稿日期:2006-11-28 修回日期:2007-02-08 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 章镇

Agrobacterium-mediated Transformation of Tomato with PvFer

YE Xia^1,2,HUANG Xiao-de¹, YAO Quan-hong³, and ZHANG Zhen^1*

(¹College of Horticulture, Nanjing Agricultural University, Nanjing，Jiangsu 210095, China; ²Henan Yellow River Gardening Limited Company, Zhengzhou,Henan 450003, China；³Shanghai Key Laboratory of Agricultural Genetic and Breeding, Shanghai 201106, China)

Received:2006-11-28 Revised:2007-02-08 Online:2007-04-25 Published:2007-04-25
Contact: ZHANG Zhen

摘要/Abstract

摘要：

利用农杆菌介导的转化方法将菜豆铁结合蛋白基因（PvFer）导入‘粉红 908’番茄基因组中。经PCR、PCR-Southern、Southern blotting检测证实，外源基因已整合到2株番茄基因组中。半定量式RT-PCR检测表明，外源菜豆铁结合蛋白基因在2株转基因植株中得到了表达。测定番茄果实和叶片中铁、锌、锰含量表明，转ferritin基因株系T1的叶片铁含量提高了174%，果实中的铁含量提高了37%，锌在果实中提高了119%，而锰在果实中的含量却降低了56%。

关键词: 番茄, 铁蛋白, 根癌农杆菌, 转化

Abstract:

In this study, the bean iron-binding protein gene (PvFer) was introduced into the genome of tomato Fenhong 908 by Agrobacterium-mediated genetic transformation. The results of PCR，PCR-Southern and Southern blotting analysis confirmed that foreign ferritin gene was integrated into the genome of two tomato plants. RT-PCR revealed that the foreign ferritin gene was expressed in the two tomato plants. Measurement of Fe, Zn, Mn contents in fruit and leaf of the transgenic tomato plants suggested that iron content in leaves of T1 transgenic line was approximately 2.7 times that of the non-transformed leaves, and iron content in fruits was increased by 37%; zinc content in fruits was increased by 119%, but manganese content in fruits of T1 transgenic line was decreased by 56%.

Key words: Tomato, ferritin, Agrobacterium tumefaciens, Transformation

中图分类号:

S 641.2

叶霞;黄晓德;姚泉洪; 章镇. 农杆菌介导菜豆铁结合蛋白基因(PvFer)转化番茄的研究[J]. 园艺学报, 2007, 34(2): 489-492.

YE Xia;HUANG Xiao-de;YAO Quan-hong;and ZHANG Zhen. Agrobacterium-mediated Transformation of Tomato with PvFer[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 489-492.

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