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园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 244-248.

• 研究论文 • 上一篇    下一篇

苹果果实酸/低酸性状的SSR分析

姚玉新1;翟 衡1*;赵玲玲1;伊 凯2;刘 志2;宋 烨1

  

  1. (1 山东农业大学园艺科学与工程学院, 泰安271018; 2 辽宁省果树科学研究所, 熊岳115214)
  • 收稿日期:2005-05-09 修回日期:2005-08-30 出版日期:2006-04-25 发布日期:2006-04-25

Analysis of Apple Fruit Acid /Low-acid Trait by SSR Marker

Yao Yuxin1;Zhai Heng1*;Zhao Lingling1; Yi Kai2;Liu Zhi2;Song Ye1

  

  1. (1College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China; 2Liaoning Fruit Research Institute, Xiongyue 115214, China)
  • Received:2005-05-09 Revised:2005-08-30 Online:2006-04-25 Published:2006-04-25

摘要: 以91株‘东光’和‘富士’的正反交F1 代群体为试材, 测定了成熟果实可滴定酸含量和果实不同发育阶段苹果酸含量的变化, 从表型上分析了苹果酸的遗传规律。利用140对共显性遗传的SSR引物对高酸和低酸个体群体分离分析, 筛选到一个与果实酸/低酸性状连锁的标记SDY085, 遗传距离为8.89 cM, 表型分析和标记分析都表明苹果果实酸/低酸性状由一对主效基因Ma/ma控制, 并且Mama为完全显性, 而酸个体中苹果酸的连续分布则是加性多基因作用的结果。

关键词: 苹果, 酸/低酸性状, SSR标记, 苹果酸

Abstract:

It is necessary for app le genomic research and breeding to find outmalic acid genetic characteristics. In this research, SSR marker linked to acid /low-acid trait in app le fruitwas identified from 140 SSR primer pairs, materialed by 91 F1 population hybrid from the intraspecific cross between apple cultivar‘Dongguang’ and ‘Fuji’. Of 140 SSR primer pairs, only p rimer SDY085 produced a polymorphic band linked to acid trait, and the linkage distance was 8.89 cM. Also, the titrated acid and malic acid in different developmental stages were determined. The SSR marker analysis, coupled with the change of the total acid and malic acid contents, revealed that acid/low-acid trait was governed by a major gene and acid trait was complete dominant.

Key words: Apple, Malus ×domestica Borkh., Acid/low-acid trait, SSR marker, Malic acid