蝴蝶兰叶片离体培养胚状体的发生及组织学观察

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 431-436.

蝴蝶兰叶片离体培养胚状体的发生及组织学观察

崔广荣^1,2;侯喜林^1* ;张子学²;张从宇²;胡能兵²;刘跃成²

（¹南京农业大学园艺学院,南京 210095;²安徽科技学院植物科学系,安徽凤阳 233100）

收稿日期:2006-08-23 修回日期:2007-03-12 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 侯喜林

Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations

CUI Guang-rong^1,2,HOU Xi-lin^1*,ZHANG Zi-xue²,ZHANG Cong-yu²,HU Neng-bing²,and LIU Yue-cheng²

(¹College of Horticulture Nanjing Agricultural University,Nanjing 210095，China;²Plant Science Department of Anhui Science and Technology University, Fengyang,Anhui 233100,China )

Received:2006-08-23 Revised:2007-03-12 Online:2007-04-25 Published:2007-04-25
Contact: HOU Xi-lin

摘要/Abstract

摘要： 以蝴蝶兰试管苗幼叶为外植体，以1/2MS为基本培养基，研究了不同浓度6-苄基腺嘌呤（6-BA）、腺嘌呤硫酸盐（Ad）、萘乙酸（NAA）及其组合对胚状体发生的影响，同时也探讨了蔗糖浓度及椰汁添加量对胚状体形成的影响。结果表明：细胞分裂素6-BA对胚状体的诱导效果较Ad好，NAA与二者配合使用可提高胚状体的发生率，其最佳浓度组合为6-BA 4.0 mg/L +Ad 2.0~4.0 mg/L+NAA 1.0~2.0 mg/L。蔗糖20~40 g/L+椰汁200 ml/L有利于胚状体的形成。组织学观察表明，胚状体起源于叶片气孔附近的上表皮细胞或上表皮下方的叶肉组织细胞，为单细胞起源，其发育过程与一般植物胚状体发生发育特征相似，最终发育成为类原球茎。

关键词: 蝴蝶兰, 叶片, 胚状体, 组织培养, 组织学观察, 原球茎

Abstract: The leaves of phalaenopsis were used as explants to culture in the 1/2MS basic medium with different kinds of plant growth regulators for somatic embryos induction. The effects of different concentrations of three kinds of plant growth regulators 6-BA, Ad, NAA and their combinations on somatic embryogenesis were studied in vitro culture. Meanwhile, the influences of the concentrations of sucrose (w/v) and coconut water (v/v) on somatic embryogenesis were studied too. The results showed that the effects of cytokinins N6-benzyl adenine (6-BA) was better than adenine sulphate (Ad) in embryoid induction. The auxin а-naphthaleneacetic acid (NAA) combined with given concentrations of 6-BA and Ad could effectively raise the frequency of somatic embryogenesis. The optimum plant growth regulator combination for somatic embryogenesis was 6-BA 4.0 mg/L +Ad 2.0-4.0 mg/L+NAA 1.0-2.0mg/L. 20-40 g/L sucrose (w/v) and 200 ml/L coconut water (v/v) added in the medium was benefit for somatic embryogenesis. The results of histological observations indicated that somatic embryos originated from upper epidermis and mesophyll single cell nearby stoma. The course of somatic embryos development was similar to other plants. Finally, the somatic embryos became protocorm-like body.

Key words: Phalaenopsis, Leaf, Somatic embryo, Plant tissue culture, Histological observation, Protocorm-like body

中图分类号:

S 682.31

崔广荣;侯喜林;张子学;张从宇;胡能兵;刘跃成. 蝴蝶兰叶片离体培养胚状体的发生及组织学观察[J]. 园艺学报, 2007, 34(2): 431-436.

CUI Guang-rong;HOU Xi-lin;ZHANG Zi-xue;ZHANG Cong-yu;HU Neng-bing;and LIU Yue-cheng. Efficient Somatic Embryogenesis from Leaf Explants of Phalaenopsis in vitro Culture and Histological Observations[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 431-436.

[1]	蒋彧, 涂勋良, 何俊蓉. 国兰叶色突变体叶片差异表达基因分析[J]. 园艺学报, 2023, 50(2): 371-381.
[2]	陆楚桥, 高洁, 谢琦, 魏永路, 金建鹏, 杨凤玺, 朱根发. 蝴蝶兰新品种‘金象’[J]. 园艺学报, 2022, 49(S1): 139-140.
[3]	蒋亚君, 陈佳佳, 谭彬, 郑先波, 王伟, 张郎郎, 程钧, 冯建灿. 桃PpIDD11调控花发育的功能研究[J]. 园艺学报, 2022, 49(9): 1841-1852.
[4]	郭燕, 张树航, 李颖, 张馨方, 王英杰, 王广鹏. 燕山板栗种质资源叶片表型性状多样性研究[J]. 园艺学报, 2022, 49(8): 1673-1688.
[5]	吴婷, 贾瑞冬, 杨树华, 赵鑫, 于晓南, 国圆, 葛红. 蝴蝶兰多倍体育种研究进展与展望[J]. 园艺学报, 2022, 49(2): 448-462.
[6]	张文心, 曹雪莹, 周锦, 周世明, 张别鱼, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵3号’[J]. 园艺学报, 2021, 48(S2): 2909-2910.
[7]	曹雪莹, 张文心, 周锦, 周世明, 张别鱼, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵4号’[J]. 园艺学报, 2021, 48(S2): 2911-2912.
[8]	官敏华, 曹雪莹, 张文心, 叶宇壮, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵5号’[J]. 园艺学报, 2021, 48(S2): 2913-2914.
[9]	邓怿, 张文心, 曹雪莹, 房林, 李琳, 吴坤林, 刘海涛, 曾宋君, . 朱顶红新品种‘双艳’[J]. 园艺学报, 2021, 48(S2): 2915-2916.
[10]	钟灶发, 张利娟, 高思思, 彭婷. 干旱胁迫下4种柑橘砧木叶片细胞学特征及抗旱性比较[J]. 园艺学报, 2021, 48(8): 1579-1588.
[11]	陈祖民, 校诺娅, 张艳霞, 史晓敏, 郭帅奇, 高虎, 王振平. 水分胁迫对‘玫瑰香’葡萄果实挥发性化合物及相关基因表达的影响[J]. 园艺学报, 2021, 48(5): 883-896.
[12]	曾泽湘, 肖显梅, 谭小丽, 范中奇, 陈建业. 菜薹BrWRKY57的特性及其对BrPPH1和BrNCED3的调控[J]. 园艺学报, 2021, 48(3): 518-530.
[13]	贾兵, 郭国凌, 王友煜, 叶振风, 刘莉, 刘普, 衡伟, 朱立武. ‘黄金梨’缺铁黄化叶片受GA₃诱导复绿的机理研究[J]. 园艺学报, 2021, 48(2): 254-264.
[14]	彭芳芳, 龙治坚, 魏召新, 李勋兰, 罗友进, 韩国辉. 樱桃种质SCoT分子标记与叶片表型性状关联分析[J]. 园艺学报, 2021, 48(2): 325-335.
[15]	胡伟芳, 何兆桓, 钟广炎, 钟云, 张木, 曾继吾, 曾鑫年, 彭埃天, 张宝鑫, 赖多, 李国良. 不同种类和树龄柑橘园土壤和叶片微量元素含量与丰缺评价[J]. 园艺学报, 2021, 48(12): 2325-2335.