关键词: 百合, LfMADS基因, 载体构建, 功能分析

Abstract: In order to analyse the function of LfMADS1 and LfMADS3 from lily, their sense and antisense plant expression vectors were constructed by inserting into the pBin438 plasmid vector, and then transformed into tobacco by Agrobacterium tumefaciens LBA4404.The results of PCR and PCR-Southern for transgenic plants showed that the foreign genes were introduced into the tobacco genome. Ectopic expression of antisense 35S::LfMADS1 in tobacco produced one of stamen truncated with anther; while the transformed tobacco with sense 35S::LfMADS1 generated a mutant in which partial sepal was converted into petal. One of the transgenic tobacco with antisense 35S::LfMADS3 showed novel phenotype by converting partial bracts into petal, another one had a flower short of one stamen; while the transgenic tobacco with sense 35S::LfMADS3 were morphologically indistinguishable from wildtype. The preliminary results suggested that LfMADS1 belongs to B function gene, and LfMADS3 is a SEP-like gene. In comparison to the expression of other flower organ identity genes in model plants, a different gene expression pattern could be involved in Lilium.

Key words: Lilium, LfMADS, Construction of expression vector, Function analysis