大花蕙兰遗传多样性及亲缘关系的AFLP分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 417-424.

大花蕙兰遗传多样性及亲缘关系的AFLP分析

朱根发^1,2;李冬梅¹;郭振飞^2*

(¹ 广东省农业科学院花卉研究所, 广州510640; ²华南农业大学生命科学学院, 广州510642)

收稿日期:2006-09-01 修回日期:2006-11-27 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 郭振飞

Genetic Diversity and Relationship of Hybrid Cymbidium Based on AFLP Marker

ZHU Gen-fa^1,2,LI Dong-mei¹,and GUO Zhen-fei^2*

(¹Floricultural Research Institute of Guangdong Academ y of Agricultural Sciences, Guangzhou 510640, China; ²College of Life Science, South China Agricultural University, Guangzhou 510642, China)

Received:2006-09-01 Revised:2006-11-27 Online:2007-04-25 Published:2007-04-25
Contact: GUO Zhen-fei

摘要/Abstract

摘要： 对来源于日本、韩国和美国的42个大花蕙兰品种和两个国产兰属原生种进行了遗传多样性和
亲缘关系的AFLP分析, 9对多态性引物在50～500 bp内共扩增出1 597条带, 其中多态性带1 565条, 多态性比率9810%。单引物对扩增的带数156～193条, 平均每对引物扩增带数177条。42个品种具特征带或缺失带。大花蕙兰品种间的遗传多样性丰富, 品种间的相似系数0.3399 ～0.8223, 平均相似系数0.5783。UPGMA聚类结果将供试品种分为4大类, 与根据花枝类型或花径大小、花色等形态指标分类的结果相吻合, 同一产地来源甚至同一育种公司选育出的品种能基本上聚类在一起, 反映出了品种间的亲缘关系。

关键词: 兰科, 大花蕙兰, AFLP, 遗传多样性, 亲缘关系

Abstract: The genetic diversity and relationship of 42 cultivars of hybrid Cymbidium introduced from Japan, Korea and USA, and 2 wild species from China (C. aloifolium and C. f loribundum ) were evaluated using AFLP molecularmarkers in this study. Among the 1 597 AFLP bands obtained from nine selective p rimer
pairs, 1 565 (98.0% ) were polymorphic. The average number of DNA bands per p rimer pairwas 177, with
a range of 156 - 193. All the cultivars have their characteristic bands and can be identified based on the AFLP markers. Genetic similarity based on dice coefficients among the cultivars ranged from 0.3399 to 0.8223, with an average of 0.5783. The cultivars could be divided into four group s based on the UPGMA dendrogram, and this division is in consistence with the morphological classification based on the type of flower inflorescence,
flower size or flower color. Cultivars originated from the same country or the same breeding company could be clustered together in the AFLP dendrogram and this rep resents the genetic relationship of the cultivars.

Key words: Ochidaceae, Hybrid Cym bidium, AFLP, Genetic diversity, Genetic relationship

中图分类号:

S 682.31

朱根发;李冬梅;郭振飞. 大花蕙兰遗传多样性及亲缘关系的AFLP分析[J]. 园艺学报, 2007, 34(2): 417-424.

ZHU Gen-fa;LI Dong-mei;and GUO Zhen-fei. Genetic Diversity and Relationship of Hybrid Cymbidium Based on AFLP Marker[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 417-424.

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