MAR序列介导野苋菜凝集素基因在白菜中的表达

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 381-386.

MAR序列介导野苋菜凝集素基因在白菜中的表达

邓智年^1,2*;魏源文^1,2;吕维莉¹;李杨瑞¹

(¹广西农业科学院广西作物遗传改良生物技术重点开放实验室, 南宁530007; ² 广西大学农学院, 南宁530004)

收稿日期:2006-08-14 修回日期:2007-01-25 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 邓智年

Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage

DENG Zhi-nian^1,2*, WEI Yuan-wen^1,2, LüWei-li¹,and LI Yang2rui¹

(¹Key Laboratory of Crop Genetic Im provem ent and Biotechnology of Guangxi, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ²Agricultural College, Guangxi University, Nanning 530004, China )

Received:2006-08-14 Revised:2007-01-25 Online:2007-04-25 Published:2007-04-25
Contact: DENG Zhi-nian

摘要/Abstract

摘要： 以‘丰顺’白菜带柄子叶为转化受体, 用带有MAR (Matrix Attachment Region) 和不带MAR的两种植物表达载体进行农杆菌介导转化野苋菜凝集素基因(Amaranthus viridis L. agglutinin, AVA ) 获得转基因的抗蚜小白菜。分析MAR序列介导对转基因表达的影响。表明利用MAR序列介导AVA 基因表达,获得转基因植株的数量比对照提高29.63%; 转AVA基因白菜对桃蚜(Myzus persicae) 的群体发展有一定的抑制作用, 平均抑制率为55.8%; MAR序列介导AVA 基因表达的转基因植株中, 该基因的表达水平比对照高, 并且不同转基因单株间AVA基因表达差异比对照小。

关键词: 白菜, MAR, 野苋菜凝集素基因, 桃蚜, 转基因表达

Abstract: The cotyledonswith petiole as acceptors, Chinese cabbage genetic transformation system mediated by Agrobacterium tum efaciens was set up and Amaranthus viridis L. agglutin in (AVA ) transformed plants resistanting to Myzus persicae were acquired. The effects of MAR (matrix attachment region) on AVA gene exp ression in transgentic Chinese cabbage were studied with two different p lant exp ression vectors. The number of transgenic plants was 29163% higher than that of the control. The bioassay of Myzus persicae revealed that transgetic plants showed more or less enhanced resistance and inhibition to population, and the average inhibition rate was 55.8%. Among the transgetic plants that AVA gene were mediated by MAR, the expression level of AVA gene was higher than that of the control, and the expression variance of AVA gene among transgetic p lantswas smaller as well.

Key words: Chinese cabbage, Matrix attachment regions, AVA gene, Myzus persicae, Transgene expression

中图分类号:

S 634.3

邓智年;魏源文;吕维莉;李杨瑞. MAR序列介导野苋菜凝集素基因在白菜中的表达[J]. 园艺学报, 2007, 34(2): 381-386.

DENG Zhi-nian;WEI Yuan-wen;LüWei-li;and LI Yangrui. Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 381-386.

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