接种时期和培养基对无核葡萄胚挽救的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 329-332.

接种时期和培养基对无核葡萄胚挽救的影响

郭修武¹;郭印山^1,2;张海娥¹;李轶晖¹; 李成祥¹

(¹沈阳农业大学园艺学院, 沈阳110161; ²华南农业大学园艺学院, 广州510642)

收稿日期:2006-09-01 修回日期:2007-02-16 出版日期:2007-04-25 发布日期:2007-04-25

Effects of Culture Medium and Inoculating Date on Embryo Rescue of Seedless Grape

GUO Xiu-wu¹, GUO Yin-shan^1,2, ZHANG Hai-e¹, L I Yi-hui¹, and LI Cheng-xiang¹

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; ²College of Horticulture, South China Agricultural University, Guangzhou 510642, China)

Received:2006-09-01 Revised:2007-02-16 Online:2007-04-25 Published:2007-04-25

摘要/Abstract

摘要： 以4个组合康能无核×金星无核、雷明无核×金星无核、金星无核自交、雷蒙无核自交为试
材, 采用L25 (55 ) 正交设计, 研究了接种时间和培养基种类对无核葡萄胚挽救的影响。结果表明, 影响无核葡萄胚挽救最重要的因素是接种时期, 其他因素影响程度因组合而不同, 最佳接种时期在花后45～60 d,最高出苗率13.64%～75.23%。对胚珠进行一定处理有助于促进胚珠的萌发出苗, 其中横切处理与低温处理可以显著提高胚珠萌发出苗率。

关键词: 无核葡萄, 胚挽救, 影响因素

Abstract: Four combinations of Canadice ×Venus Seedless, Remaily Seedless ×Venus Seedless, Selfing of Venus Seedless and selfing of Lakemont Seedless were used as test materials in this experiment. This
experimentmainly studied the influence of inoculating time and different kinds ofmedia on the effect of embryo
rescue of seedless grape by the orthogonal experiment design of L₂₅ (5⁵ ). Results showed that the most important factor influencing the embryo rescue of seedless grape was the inoculating time, and the influence level of
other factorswas different among combinations. The op timum inoculating time of the embryo was 45 - 60 days after blooming and the seedling rate reached 13.64% - 75.23%. It also showed that certain treatments of ovule increased the germination rate of ovule, among them the treatment of crosscut and low temperature increased the germination rate of ovule significantly.

Key words: Seedless grape, Embryo rescue, Influence factors

中图分类号:

S 663.1

郭修武;郭印山;张海娥;李轶晖;李成祥. 接种时期和培养基对无核葡萄胚挽救的影响[J]. 园艺学报, 2007, 34(2): 329-332.

GUO Xiu-wu;GUO Yin-shan;ZHANG Hai-e;L I Yi-hui;and LI Cheng-xiang. Effects of Culture Medium and Inoculating Date on Embryo Rescue of Seedless Grape[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 329-332.

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