关键词: 枸杞, Lb_PPR1, 生物信息, 表达模式

Abstract: The pentatricopeptide repeats（PPR）proteins involve in post-transcriptional modifications and editing of specific single-stranded RNAs in the nucleus and organelles via their localization in various organelles，and they play important roles in series events of plant growth and development. In this study，Lb_PPR1 gene was cloned from the fertile line‘Ningqi 1’and the male-sterile line‘Ningqi 5’. Bioinformatics analysis was used to predict the physico-chemical properties，subcellular localization，conserved domain，protein structure and phylogenetic evolution of Lb_PPR1 encoded protein. The results indicated that Lb_PPR1 gene contained a 1 977 bp open reading frame encoding 658 amino acids in both ‘Ningqi 1’and ‘Ningqi 5’，but there were 20 bp and 14 aa differences between the two cultivars. Lb_PPR1 protein was predicted to have 14 tandem repeats，belonged to PLS family，and its subcellular localization was in cell membrane and cytoplasm. Secondary and tertiary structure predictions showed that Lb_PPR1 protein was dominated by α-helix. Phylogenetic analysis showed that Lb_PPR1 had the closest relationship with PPR in Capsicum annuum and Nicotiana attenuate. Quantitative real-time PCR（qRT-PCR）was performed to determine the expression pattern of Lb_PPR1 gene in different organs and different developmental stages of anther. The results showed that Lb_PPR1 was expressed in different tissues of Lycium barbarum，but highest in the fertile line’s flower buds，its expression in the male-sterile line‘Ningqi 5’were all significantly lower than the fertile line‘Ningqi 1’. The above results indicate that Lb_PPR1 gene maybe related to the development of flower organs in Lycium barbarum.

Key words: Lycium barbarum, Lb_PPR1, bioinformatics analysis, gene expression pattern