关键词: 津田芜菁, 14-3-3, 基因克隆, 胁迫, 表达

Abstract: In this study，we cloned the general regulatory factor 14-3-3 gene from Brassica rapa subsp. rapifera‘Tsuda’，which was named Br14-3-3 with the accession number of MK896872 in the NCBI GenBank. The full cDNA sequence of the Br14-3-3 gene was 1 113 bp，cotaining an open reading frame of 774 bp encoding a protein of 257 amino acids. We determined the expression of the Br14-3-3 gene in different tissues and under different abiotic stress conditions，such as extreme temperature，dehydration，adverse osmosis，abscisic acid（ABA）and salt stress by quantitative-PCR analysis. The results demonstrated that the highest expression levels of the Br14-3-3 gene could be reached in the flower of Brassica rapa subsp. rapifera‘Tsuda’，followed by the seedling. The expression of the Br14-3-3 gene was upregulated under aforementioned stress conditions except for low temperature，suggesting that the Br14-3-3 gene may play a role in response of abiotic stresses.

Key words: Brassica rapa, 14-3-3, gene cloning, abiotic stress, gene expression