关键词: 葡萄, LIM基因家族, 生物信息学分析, qRT-PCR, 非生物胁迫

Abstract: In order to clarify the expression characteristics of LIM gene family in grape，the bioinformatics analysis was carried out，and then the gene expression levels of LIM family under different stress were detected by qRT-PCR using Vitis vinifera‘Pinot Noir’plantlets as experimental material. The results showed that the LIM gene family of grape had 14 members and could be divided into four subgroups. Through the analysis of the selective pressure between grape genes and Arabidopsis genes，there was a passivation selection relationship between the two. Multiple sequence alignments revealed that the members of this family contained 1–2 conserved domains of zinc finger structure. The conserved motif results showed that all genes contained motif2. The analysis of gene structure showed that VvLIM contains 4–15 exons. Of the 14 genes，11 interacted with encoded protein. There were a large number of photoinduced elements and stress related elements such as salicylic acid in this gene family. The microarray data showed that the highly expressed genes mainly existed in the growing tissues. Compared with the control，the expression levels of VvLIM1 under 100 mmol ? L-1NaCl，10% PEG，and 50 mg ? L-1 SA stresses were significantly up-regulated by qRT-PCR analysis，and kept increasing with the treatment time.

Key words: grape, gene family of LIM, bioinformatics analysis, qRT-PCR, abiotic stress