关键词: 毛葡萄, 欧洲葡萄, 芪合酶, 白藜芦醇, 白粉病, 抗病性, 转基因

Abstract: Two complete open reading frames of VqSTS11 and VqSTS23，were respectively isolated from Vitis quinquangularis‘Danfeng-2’using homologous cloning technique and further for overexpression vector construction. Meristematic bulk（MB）of cultivated Vitis vinifera‘Thompson Seedless’，were induced through organogenesis and further for Agrobacterium-mediated transformation to obtain transgenic plants. After PCR detection and Western blot identification，5 transgenic lines of VqSTS11 and 3 transgenic lines of VqSTS23 were verified，respectively. Transgenic plants and non-transgenic plants were artificially inoculated with powdery mildew. Microscopic observation demonstrated that the hyphae growth in transgenic lines were slower than those in non-transgenic plants and spore germination was inhibited in transgenic plants. The disease resistance of transgenic plants was further analyzed by qRT-PCR and HPLC. The results displayed that not only the expression of STSs in transgenic plants increased，and the expression of disease resistance-related genes was up-regulated，but also stilbene compounds in the transgenic plants were accumulated. The expression product of‘Danfeng-2’VqSTS11 and VqSTS23 could tolerate powdery mildew. Therefore，‘Danfeng-2’could be used to improve the disease tolerance and provide disease- tolerant candidate genes for European grape cultivars，which could be served as a breeding resources for disease-tolerant breeding.

Key words: Vitis quinquangularis, Vitis vinifera, stilbene synthase, resveratrol, powdery mildew, disease resistance, transgenic