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园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 186-189.

• 研究简报 • 上一篇    下一篇

连香树离体快繁初步研究

麦苗苗;石大兴;王米力;廖 静;韩 珊
  

  1. (四川农业大学林学园艺学院, 雅安625014)
  • 收稿日期:2005-06-20 修回日期:2005-07-21 出版日期:2006-02-25 发布日期:2006-02-25

A Preliminary Study on the in Vitro Culture of Cercidiphyllum japonicum Sieb. et Zucc.

Mai Miaomiao; Shi Daxing;Wang Mili;Liao Jing; and Han Shan

  

  1. (College of Forestry and Horticulture, Sichuan Agricultural University, Ya'an 625014, China)
  • Received:2005-06-20 Revised:2005-07-21 Online:2006-02-25 Published:2006-02-25

摘要: 连香树为我国珍稀濒危树种, 具有较高的经济价值和观赏价值。本文研究了3年生连香树(Cercidiphyllum japonicum Sieb. et Zucc. ) 带芽茎段的离体培养。筛选出最佳培养基: (1) 腋芽诱导培养基: MS +NAA 0.01 mg·L - 1 ; (2) 丛生芽诱导培养基: MS +BA 1.0 mg·L - 1 ; (3) 丛生芽增殖培养基:MS +BA 2.0 mg·L - 1 + 2,4-D 0.01 mg·L - 1 ; (4) 生根培养基: 1 /2MS + IBA 1.0 mg·L - 1

关键词: 连香树, 离体培养

Abstract: Cercidiphyllum japonicum is an endangered plant in China, possesses the high economic and appreciatory value. This papermainly dealt with the study on shoot organogenesis culture in vitro culture from sprout explants of Cercidiphy llum japonicum. The results showed that the best media for various stages were as follows: (1) Axially bud induction medium: MS +NAA 0.01 mg·L - 1 ; (2) Clump shoot induction medium: MS +BA 1.0 mg·L - 1 ; (3) Clump shoot regeneration medium: MS + BA 2.0 mg·L - 1 + 2,4-D 0.01 mg·L - 1 ; (4)Rooting medium: 1/2MS + IBA 1.0 mg·L - 1.

Key words: Cercidiphyllum japonicum, In vitro culture