关键词: 药用蒲公英, 温度胁迫, 内参基因, 表达分析

Abstract: In order to research the optimal reference genes and the responsive genes expression of Taraxacum officinale under different temperature stress，the leaves of Taraxacum officinale that were treated for 0，3，6，12，24 and 48 h under low temperature（4 ℃）and high temperature（38 ℃） were used as material. 18S，EF1α，TUB，40S，GAPDH，β-actin，ACT11，TUA，60S and SKIP were selected as candidate reference genes，RT-qPCR technology，geNorm，NormFinder and BestKeeper were taken advantage of evaluating the stability. Utilizing the optimal selected reference genes analyzed the expression level of 12 temperature-responsive genes（AP2/ERF，Dof，ICE1，MYB，bZIP，NTL6，HSF，Gols，HSP，NAC，XCT，WRKY）. The results showed that，the optimal reference genes were 18S and GAPDH under different temperature stresses. The expression levels of AP2/ERF，HSF，Glos，HSP，NAC，XCT were firstly up-regulated and then down-regulated during low and high temperature stress. Changes of bZIP and NTL6 were undulant under temperature stresses. Dof，ICE1 and MYB were firstly up-regulated and then down-regulated under low temperature stress，but down-regulated continuously under high temperature. The expression of WRKY under high temperature stress was much higher than that under low temperature stress. Based on the genes expression，it was speculated that the response mechanism of Taraxacum officinale were different under low temperature and high temperature stress. AP2/ERF and Dof mainly responded to low temperature stress，and HSF，XCT and WRKY mainly played a role in high temperature stress.

Key words: Taraxacum officinale, temperature stress, reference gene, expression analysis