薰衣草叶片高频再生体系的建立

园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 182-185.

薰衣草叶片高频再生体系的建立

许耀祖^{1, 2, 3} ;韦彦余¹;王晓军^1*;赵民安¹;赵海清¹

(¹中国科学院新疆理化技术研究所, 乌鲁木齐830011;² 中国科学院研究生院, 北京100830; ³浙江大学原子核农业科学研究所, 杭州310029)

收稿日期:2004-12-06 修回日期:2005-05-18 出版日期:2006-02-25 发布日期:2006-02-25

Establishment of High-frequency Regeneration System from Leaf Explants ofLavandula angustifolia ‘Munstead’

Xu Yaozu1, 2 , Wei Yanyu1 , Wang Xiaojun1 , ZhaoMinpan1 , and Zhao Haiqing1

(¹Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China; ²Graduate School of Chinese Academy of Sciences, Beijing 100830, China; ³Institute of Nuclear-agricultural Sciences, Zhejiang University, Hangzhou 310029, China)

Received:2004-12-06 Revised:2005-05-18 Online:2006-02-25 Published:2006-02-25

摘要/Abstract

摘要： 以薰衣草叶片为外植体进行了离体再生研究。结果表明: 叶片愈伤组织诱导最佳的培养基是MS + 2,4-D 0.1 mg/L + 6-BA 0.5 mg/L, 诱导率高达100%; 液体悬浮—固体培养芽分化率达92.5% , 芽数/愈伤组织达6.6; 正交试验筛选出芽增殖最佳培养基为MS +NAA 2.0 mg/L + 6-BA 0.5 mg/L + IAA 1.0 mg/L, 其增殖系数高达8.7; 在芽增殖培养基上可直接生根, 生根率达100%。

关键词: 薰衣草, 液体悬浮—固体培养, 再生植株

Abstract: The regeneration system of Lavandula angustifolia ‘Munstead’ in vitro was studied with leaves as explants, and the results showed the optimal medium for callus induction was MS + 2,4-D 0.1 mg/L + 6-BA 0.5 mg/L with the induction frequency of 100%. Liquid suspension solid culture can achieve adventitious bud differentiation rate of 92.5% and 6.6 bud per callus. Best bud multip lying medium which was MS medium supplemented with NAA 2.0 mg/L and 6-BA 0.5 mg/L and IAA 1.0 mg/L was sifted through orthogonal experiment with multip lying coefficient of 8.7. The rooting of rootless bud could be accomplished directly in the bud multip lying medium with rooting frequency of 100%.

Key words: Lavandula angustifolia, Liquid suspension-solid culture, Regenerated plantlet

许耀祖;;;韦彦余;王晓军;赵民安;赵海清. 薰衣草叶片高频再生体系的建立[J]. 园艺学报, 2006, 33(1): 182-185.

Xu Yaozu;;Wei Yanyu;Wang Xiaojun;ZhaoMinpan;and Zhao Haiqing. Establishment of High-frequency Regeneration System from Leaf Explants ofLavandula angustifolia ‘Munstead’[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 182-185.

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