关键词: 茶树, 脱氢抗坏血酸还原酶, 序列特性, 逆境响应, 表达分析

Abstract: Using RT-PCR technology，the CsDHAR gene encoding dehydroascorbate reductase was cloned from the cDNA of Camellia sinensis‘Longjing 43’. CsDHAR gene was 639 bp in length，encoding 212 amino acids，belonging to glutathione transferase superfamily which is highly conservative. Phylogenetic analysis showed that CsDHAR protein was closely related to Arabidopsis DHAR1 and DHAR2. Among different species，CsDHAR protein was closely related to Populus trichocarpa and P. euphratica. Physicochemical analysis showed that CsDHAR protein was a hydrophilic protein. Structural analysis showed that CsDHAR protein has a typical functional domain，and the tertiary structure is mainly composed of alpha-helix and irregular curl. Quantitative real-time PCR analysis showed that CsDHAR expression levels were not significantly different at different growth stages in leaves of tea plant. CsDHAR gene expression profiles of C. sinensis‘Longjing 43’and‘Anji Baicha’were detected and analyzed by qRT-PCR.‘Longjing 43’had the highest expression level in 4 h under low and high temperature treatments，and the highest expression level in 24 h under drought and high salt treatments. The expression level of‘Anji Baicha’was the highest in 4 h under high temperature and high salt treatments，and the highest expression level in 2 h under drought treatment. Under different abiotic stress treatments，CsDHAR was induced，indicating that the gene was involved in the regulation process of stress response in tea plant.

Key words: Camellia sinensis, dehydroascorbate reductase, sequence characteristic, stress response, expression analysis