关键词: 梨, CRK, 基因家族, 腐烂病, 生物信息分析

Abstract: The Cysteine-rich receptor like kinases（CRKs）took important regulatory roles during plant：PF01657）and kinase（Pfam：PF00069），a genome-wide identification of CRKs in pear（Pyrus spp.）genome were performed. In addition，the physical and chemical characteristics，evolutionary characteristics，subcellular location，chromosomal distribution，cis-acting regulatory element（cis-element）and expression patterns were analyzed. A total of 32 CRKs were obtained，with the amino acid size from 440 to 1 217 aa，molecular weight of 48.90–137.02 kD，and isoelectric point of 5.31–8.59. CRKs were predicted mainly located on plasma membrane. According to evolutionary analysis，a total of 156 CRKs from 5 plant species，including pear，Arabidopsis thaliana，apple，tomato and rice，could be divided into 6 subgroups. Pear CRKs were mainly distributed in subgroup Ⅳ，Ⅴand Ⅵ. In pear CRKs，5 tandem duplicated gene clusters，including 20 members，were found. For all the family members，a large number of cis-elements responsive to hormone and stress were discovered in promote regions. After inoculated Valsa canker pathogen Valsa pyri（Vp），8 and 10 CRKs were differentially expressed in Pyrus betulifolia （resistant）and‘Zaosu’pear（Pyrus bretschneideri，susceptible），expression of 6 members were changed in both two germplasm. Among these，Pbr001477.1 and Pbr000205.4 were up-regulated in two germplasm，but other genes exhibited distinct expressions between two germplasm.

Key words: pear, CRK, gene family, Valsa canker, bioinformatic analysis