关键词: 杏, CCD1, CCD4, 启动子, 功能分析

Abstract: The promoters of pPaCCD1 and pPaCCD4 were cloned by genomic walking method using the CCD1 and CCD4 gene fragments in the transcriptome database. Sequencing showed that the lengths of pPaCCD1 and pPaCCD4 promoter were 2 233 bp and 1 838 bp，respectively. The cis-acting elements of promoter were analyzed and predicted using PlantCare databases. The results showed that two promoters contain multiple cis-acting elements associated with light signal，abscisic acid，and methyl jasmonate. In addition，the PaCCD1 promoter contains gibberellin，low temperature and transcription enhancer elements. The results suggested that the expression of PaCCD1 and PaCCD4 may be regulated by light signaling，plant hormone and stress. To further define two promoters’ core regions，two promoter-reporter vectors were respectively constructed based on structural characters of promoters and distributions of controlling elements in promoter using the promoter deletion mutation，and the vectors were fused to GUS gene and then were transformed into tobacco leaves. The efficient cis-acting element of PaCCD1 was included in–2 174 to–1 700 bp，–1 700 to–1 200 bp，–1 200 to–600 bp，while the efficient cis-acting element of PaCCD4 was included in–1 740 to–1 200 bp，–1 200 to–600 bp.The GUS enzyme activity of PaCCD1 and PaCCD4 was gradually weakened with the deletion of the promoter fragment. These results revealed that the core regions of the PaCCD1 and PaCCD4 promoter were within –1 200 to 2 174 bp and–1 200 to–1 740 bp，respectively.

Key words: apricot, CCD1, CCD4, promoter, function analysis