关键词: 番茄, 拮抗细菌, 枯草芽孢杆菌, 番茄匍柄霉叶斑病, 防治效果

Abstract: Gray leaf spot is one of major diseases on tomato. At present，chemical control was the only way against the disease. So it has a significant meaning to screen and use biocontrol bacteria to deal with the disease. A bacterial strain，named ZF161，isolated from rhizosphere soil of tomato in a continuous cropping system，exhibited a high antagonistic effect on gray leaf spot of tomato caused by Stemphylium solani Weber. The plate bioassay showed that the inhibition rate on S. solani was 70.51%. The control effect of ZF161 against tomato gray leaf spot of inoculated tomato detached leaf was 69.83%. The strain was identified as Bacillus subtilis based on morphological，biochemical and physiological analysis. The pot experiment result revealed that the control effect of ZF161 against tomato gray leaf spot was 63.27%. The antifungal activity of strain ZF161 was tested by plate confrontation method. The result showed that strain ZF161 exhibited a broad spectrum against seven pathogenic fungi. Overall，B. subtilis ZF161 is a beneficial bacteria in biocontrol of plant disease.

Key words: tomato, antagonistic bacteria, Bacillus subtilis, gray leaf spot of tomato, control effect