关键词: 菠萝, 短营养期, 突变体, DNA, AFLP

Abstract: The genetic differences between‘Hime Pineapple’（Ananas nanus）and its short-vegetative- growth-period mutant‘14-1’were analyzed by AFLP molecular marker technology. Identifying the authenticity of mutants could provide references for the molecular breeding of short-vegetative- growth-period pineapples. The young leaves of mutant‘14-1’and‘Hime Pineapple’were used as experimental objects and their leaf genomic DNAs were extracted by improved CTAB method. Totally 1 952 clear bands were obtained by 64 pairs of AFLP selective amplification primers（each pair of primers produced 30.5 bands on average），among them，DNA molecular polymorphisms were detected in 18 pairs and 76 polymorphism bands were obtained which accounted for 4.54%. The difference fragments mainly appeared between 200–500 bp and the genetic similarity coefficient was 95.39%. After selecting，recycling，cloning，sequencing and homologous analysis of 76 amplified difference bands of AFLP，two of them were homologous to the MADS-box transcription factor 6-like gene of pineapple，which indicated that the character differences between mutant‘14-1’and‘Hime Pineapple’were related to the changes of genetic material. Corresponding specific bands could be used as candidate gene segments that regulate vegetative duration or were linked with genes that regulate vegetative duration. The results could provide good reference value for exploration of the key genes of early flowering and variation mechanism of early flowering with short-vegetative-growth-period varieties.

Key words: Ananas nanus, short-vegetative-growth-period, mutant, DNA, AFLP