关键词: 月季, RhOOMT2, 启动子, 花瓣特异性表达

Abstract: Thirteen color/scent-related genes in Rosa hybrida‘Samantha’were selected，and their expression in root，stem，leaf and petal showed that RhOOMT2（orcinol o-methyltransferase 2）and RhCCD4（carotenoid cleavage dioxygenase 4）had relatively high expression in petals. RhNUDX1 had high expression in root，moderate in petal，and weak in stem and leaf. The other ten anthocyanin synthesis-related genes did not show much difference in root，stem，leaf，and petal. The promoter sequences of RhOOMT2，RhCCD4 and RhNUDX1 were got by using FPNI-PCR method，whose length was 1 601，1 539 and 1 433 bp，separately. Agrobacterium tumefaciens EHA105 carrying 35S::GUS，proRhOOMT2::GUS，proRhCCD4::GUS，or proRhNUDX1::GUS was transferred into petals of rose （R. hybrida‘Samantha’），eustoma（Eustoma grandiflorum‘Green Pelleted’），and lily（Lillium Oriental hybrida‘Siberia’）by vacuum aspiration. The GUS staining result showed that the promoter of RhOOMT2 had stronger activity than 35S in rose，eustoma and lily petals，the promoter of RhCCD4 had relatively weak activity in lily petals，and very weak activity in rose and eustoma，and the promoter of RhNUDX1 almost had no activity in three kinds of flowers. The promoter activity of RhOOMT2 and 35S was also compared in four cultivars of rose，‘Samantha’，‘Diana’，‘Champagne’，and‘Snow Mountain’，the result confirmed that the promoter of RhOOMT2 had higher activity than 35S in petals.

Key words: rose；RhOOMT2, promoter, petal-specific expression