关键词: 茶树, PAL 家族基因, 生物胁迫, 非生物胁迫, 表达分析

Abstract: Phenylalanine ammonia-lyase（PAL）plays important roles in plant stress resistance. Based on bioinformatics analyses，five and six PAL genes were identified from the genomes of Camellia sinensis var. assamica‘Yunkang 10’and Camellia sinensis var. sinensis‘Longjing 43’respectively. Homology analysis showed that the CsPAL proteins had low homology to the proteins of Arabidopsis thaliana and Populus trichocarpa，and showed different evolutionary relationship between‘Longjing 43’and‘Yunkang 10’. Transcriptome data further showed that CsPAL genes could be significantly induced under salt stress and methyl jasmonate treatment，while not for cold and drought stresses. Tissue-specific expression of PAL genes showed relatively low transcript abundance in the old leaves of tea plant，but high in young tissues and roots，and particularly the expression of CsPALa，CsPALc，CsPALe were the highest in flowers. Furthermore, qRT-PCR detection showed that the expression of six CsPAL genes were significantly up-regulated after inoculation at 24 h with Colletotrichum camelliae，Pseudopestalotiopsis camelliaesinensis and Ectropis oblique in‘Longjing 43’and new line‘2807’. The above results indicate that the PAL genes of tea plant play important roles in dealing with different stresses.

Key words: Camellia sinensis, PAL gene family, biotic and abiotic stress, expression analysis