结球甘蓝游离小孢子培养及植株再生

园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 158-160.

结球甘蓝游离小孢子培养及植株再生

方淑桂;陈文辉^*;曾小玲;朱朝辉;廖晓珍;郑学立

(福州市蔬菜科学研究所, 福州350012)

收稿日期:2005-05-23 修回日期:2005-09-12 出版日期:2006-02-25 发布日期:2006-02-25

Isolated-m icrospore Culture and Plantlet Regeneration in Cabbage ( Brassicaoleracea L. var. capitata L. )

Fang Shugui;ChenWenhui^*;Zeng Xiaoling;Zhu Chaohui;Liao Xiaozhen;and Zheng Xueli

( Fuzhou Institute of Vegetable Research, Fuzhou 350012, China)

Received:2005-05-23 Revised:2005-09-12 Online:2006-02-25 Published:2006-02-25

摘要/Abstract

摘要： 以结球甘蓝的双交种和四交种为材料, 研究了影响甘蓝游离小孢子培养的若干因素。结果表明: 基因型是影响小孢子胚状体产生的最关键因素之一; 用高出胚的材料与不易出胚的材料杂交后, 能明显提高不易出胚材料的出胚能力; 双交种比单交种容易培养出健壮的胚, 成苗率也高; 高糖培养4 d后添加低糖培养液可显著提高胚产量。

关键词: 结球甘蓝, 双交种, 游离小孢子培养

Abstract: Some factors affecting isolated2microspore culture of cabbage (Brassica oleracea L. var. capitata L. ) were studied using double-crossed and tetra-crossed hybrids as the donor plants. Results showed that the genotype was one of the most critical factors affecting embryogenesis in isolated microspore culture. The embryogenesis capacity of the low responsive genotype could be significantly improved after crossed with the genotype with high embryogenesis ability. The tetra-crossed hybrid could produce more vigorous embryos and result higher seedling rate compared with the double-crossed hybrid. The yield of embryoid could be significantly increased by adding new liquid medium with lower sucrose concentration into the original culture medium with a higher sucrose concentration after 4 days of culture.

Key words: Cabbage, Tetra-crossed hybrid, Isolated-microspore culture

方淑桂;陈文辉;曾小玲;朱朝辉;廖晓珍;郑学立. 结球甘蓝游离小孢子培养及植株再生[J]. 园艺学报, 2006, 33(1): 158-160.

Fang Shugui;ChenWenhui;Zeng Xiaoling;Zhu Chaohui;Liao Xiaozhen;and Zheng Xueli. Isolated-m icrospore Culture and Plantlet Regeneration in Cabbage ( Brassicaoleracea L. var. capitata L. )[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 158-160.

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