关键词: 柑橘, 柑橘溃疡病, GST pull-down, CsAP2-09, AP2/ERF, 互作蛋白

Abstract: CsAP2-09 is a positive transcription factor involved in the citrus bacterial canker resistance. In this study，the GST pull-down and LC–MS/MS strategies were used for the screening and network analysis of the interacting proteins of CsAP2-09. Firstly，the fusion proteins GST-CsAP2-09 were expressed and purified for the next GST pull-down. Then GST-CsAP2-09 was fixed on the GST-beads and incubated with total proteins extracted from CsAP2-09 over-expression plant. The interacting proteins of CsAP2-09 were detected by LC–MS/MS. From the annotation，GO and KEGG，17 interacting proteins were obtained，5 of which were reported to be involved in bacterial resistance，e.g. Cs3g27280（CAT）-a catalase.The interacting network was constructed，among which 14 of 17 interacting proteins were involved. Sixteen proteins were positively regulated by CsAP2-09 in the transgenic plant while 1 protein was negatively regulated.

Key words: citrus, citrus bacterial canker（CBC）, GST pull-down, CsAP2-09, AP2/ERF, interacting protein