关键词: 番茄, 长花柱, 遗传模型, 遗传率, QTL 定位

Abstract: In this study，the temperature sensitivity，inheritance and QTL（quantitative trait locus）analysis of long-style trait were performed using a tomato（Solanum lycopersicum）material，J59，which shows a long style phenotype. The main results were as follows：when the seedlings at two-leaf stage were treated with different temperature conditions，including 18/10 ℃，25/18 ℃，and 35/25 ℃ in day/night，respectively，the lengths of pistil，stamen，style，and stigma exsrtion were not significantly changed，implying that the tomato J59 is not a temperature-sensitive material. Furthermore，the six generations of genetic groups，namely，P1，P2，F1，BC1P1，BC1P2，and F2，were constructed using the J59 and M82（short style）tomatoes as parental materials. Genetic analysis indicated that the long style trait conforms to the MX2-ADI-AD model and is mainly controlled by major genes. The inheritabilities of major genes in BC1P1，BC1P2 and F2 populations are 65.51%，90.76% and 89.10%，respectively. Moreover，using 121 polymorphic SSR markers，a genetic linkage map was constructed，which consists of 12 linkage groups，and covers a total length of 2 108.71 cM in the genome with an average of 17.43 cM between the adjacent markers. The QTL analysis was performed based on the inclusive composite interval mapping method. 10 QTLs associated with long-style trait，which distribute in Chr1，Chr2，Chr3，Chr4，Chr5，and Chr12，were detected. LOD scores are ranged from 2.53 to 26.85. These QTLs can explain the phenotypic variability of 1.28%–20.22%，in which，the variabilities of 3 QTLs are higher than 10%，accounting for 30% of the total QTL.

Key words: tomato, long style, genetic model, inheritability, QTL mapping