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园艺学报 ›› 2008, Vol. 35 ›› Issue (6): 841-846.

• 蔬菜 • 上一篇    下一篇

大蒜白腐病菌产毒素培养条件的优化

张林青1,2, 程智慧1*   

  1. 1西北农林科技大学园艺学院, 陕西 杨凌 712100; 2淮阴工学院, 江苏 淮安223001)
  • 收稿日期:2008-01-30 修回日期:2008-05-13 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 程智慧

Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk. of Garlic

ZHANG lin-qing1,2 and CHENG Zhi-hui1*
  

  1. (1CoLLege of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China; 2Huaiyin Institute of Technology, Huaian, Jiangsu 223001, China )
  • Received:2008-01-30 Revised:2008-05-13 Online:2008-06-25 Published:2008-06-25
  • Contact: CHENG Zhi-hui

摘要:

采用大蒜幼苗根系生长抑制率生物检测法,研究了该病菌在不同培养基、培养时间、培养温度、培养液pH、光照和振荡培养等条件下粗毒素的活性和产量,结果表明:大蒜白腐病病菌的最佳产毒培养基为Fries液体培养基,培养温度为18.0 ℃,培养基pH值为5.0,在黑暗条件下连续振荡培养6 d时产生的粗毒素的毒性最强。

关键词: 大蒜, 白腐病菌, 培养条件, 粗毒素

Abstract: A test of crude toxin toxicity using garlic root inhibition was examined from toxin production related to culture medium, temperature, pH, culturing duration, light, dark or shaking conditions. Investigations concerned a garlic white rot pathogen, Sclerotium cepivorum Berk. Results showed that optimum conditions included using a Fries liquid medium at pH 5 and at18 oC, with successive dark periods and shaking during culturing. Production of crude toxin reached the highest level on the 6th day of culturing.

Key words: garlic, Sclerotium cepivorum Berk., culturing conditions, crude toxin

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