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园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 134-136.

• 研究简报 • 上一篇    下一篇

‘石硖’龙眼芽变系双引物RAPD鉴定的研究

王心燕1;肖 璇2 ;乔爱民1;孙 敏3

  

  1. (1 仲恺农业技术学院园艺系, 广州510225; 2 江西赣南师范学院化学与生命科学系, 赣州341000; 3 西南师范大学生命科学学院, 重庆400715)
  • 收稿日期:2005-01-25 修回日期:2005-07-22 出版日期:2006-02-25 发布日期:2006-02-25

RAPD Studies on Sport Line of‘Shixia’Longan with Double Primers

Wang Xinyan1;Xiao Xuan2;Qiao Aimin1; and Sun Min3   

  1. (1Departm ent of Horticulture, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China; 2Department of Chemistry and Life Science, Gannan Normal University, Ganzhou 341000, China; 3College of Life Science, Southwest China Normal University, Chongqing 400715, China)
  • Received:2005-01-25 Revised:2005-07-22 Online:2006-02-25 Published:2006-02-25

摘要: 以‘石硖’龙眼芽变系及其普通系(对照) 为材料, 从80对双引物中筛选出4对双引物在二者之间扩增出稳定清晰的多态性片段。4对双引物共扩增出35条带, 其中多态性带5条, 表明通过双引物扩增可成功地将‘芽变’与母株(对照) 区分开来。在此基础上对双引物产生多态性扩增的原理进行了分析。

关键词: 龙眼, 芽变, RAPD, 双引物

Abstract: A single 10 bp random oligonucleotide was often used as the primer in the standard RAPD-PCR analysis. To identify the possibility of RAPD-PCR reaction with double 10 bp primers, 80 double 10 bp oligonucleotideswere applied to the RAPD-PCR analysis between the ‘Shixia’ longan (Dimocarpus longan var. shixia Lour. ) sport line and itps normal line ( control). Four double primers which could produce clear and stability polymorphic DNA fragments were screened. Five RAPD makers have been obtained which were linked to the mutation gene of the sport line. Furthermore, the reason why the new RAPD fragments could be produced by double primers was also discussed.

Key words: Longan, Sport, RAPD, Double primers