关键词: 山茶, 叶斑病, 叶点霉菌

Abstract: A new leaf spot disease of Camellia was found in Jinhua Camellia Planting Garden，Zhejiang Province in 2016，the disease was named“green island type”leaf spot. Collection of diseased leaves，isolation and purification of pathogen，morphological characteristics，pathogenicity test and the sequence analysis of rDNA-ITS were used to confirm the pathogen of causing leaf spot. The results show that，the symptoms of artificial inoculation are in accordance with the symptoms in the field. Pathogen’s pycnidia are sphaeroid with ostiole，conidia are mostly pyriform，and usually covered by a slime layer and bearing a single apical appendage. The morphology of pycnidia and conidia is conforming to Phyllosticta. Homology comparison between the rDNA-ITS sequence of the pathogen to be tested and the related strains in the NCBI Databases was analysed. The homology was 99% between the pathogen and Phyllosticta capitalensis. The results indicated that Phyllosticta capitalensis was the pathogen of “green island type”leaf spot.

Key words: Camellia japonica, leaf spot, Phyllosticta capitalensis