关键词: 苹果, 苹果褪绿叶斑病毒, CP, 一致性, 系统发育进化分析, 重组

Abstract: In order to clarify the incidence and diversity of Apple chlorotic leaf spot virus（ACLSV）in northeast China and Inner Mongolia，a total of 165 apple leaf samples were collected from 8 sampling points in these areas and tested by reverse transcription-polymerase chain reaction. The average incidence of ACLSV among the collected samples was 60.6%，suggesting its wide spread in the surveyed regions. The fragments of the complete coat protein（CP）genes generated from 25 samples were cloned and sequenced. Pairwise comparison analysis showed that the nucleotide and amino acid sequence identities of CP genes of the 25 isolates ranged from 84.0%–99.7% and 90.7%–99.5%，respectively，while they shared the nucleotide and amino acid sequence identities with another 13 ACLSV isolates of 67.7%–99.7% and 71.0%–99.5%，respectively. The ACLSV isolates worldwide were grouped into four groups（B6，P205，SHZ and Ta Tao 5），and the 25 ACLSV isolates were located in the groups B6，P205 and SHZ. No significant evidence of recombination was found among the 25 complete CP sequences.

Key words: apple, ACLSV, CP, identity, phylogenetic analysis, recombination