关键词: 非洲菊, 花瓣, GASA家族, 基因表达

Abstract: In this study，full-length of GEG2，GEG3 and GEG4 cDNA sequences were cloned from petals of Gerbera hybrida culture‘Shenzhen 5’by using RACE，which were named GEG2，GEG3，and GEG4，respectively（GenBank accession numbers are KC620442，KC620443 and KC620444）. Temporal and spatial expression patterns of five GhGASA were analyzed. The main results are as follows：（1） none of the five genes expressed in floral organs specifically；（2） GEG2，GEG4 and PRGL were only expressed in the early petals，whereas GEG was significantly accumulated in the late petals，and GEG3 showed a monotonous expression pattern during the developmental process of petals.（3）The expression of GEG3 and PRGL was up-regulated by GA3 and down-regulated by ABA. The responses of GEG，GEG2 and GEG4 to different plant hormones were significantly different，suggesting that five GhGASA genes might participate in multiple hormone regulation processes.

Key words: Gerbera, petal, GASA, gene expression