关键词: 甜瓜, Dof, 基因家族, 进化分析, 表达特性

Abstract: In this study，we systematically investigated the members of Dof family in melon（Cucumis melo L.）based on the whole-genome sequences and elucidated their phylogenetic relationships. Responses to PEG6000，salt and chilling stresses were detected by real-time PCR method. The results showed that a total of 34 CmDof genes were identified in melon. These CmDof genes were distributed on all chromosomes except the 1st，5th and 7th. Two pairs of tandem duplicated genes were identified，and 11 pairs of segmental duplication genes were observed. The number of exons ranged from 1 to 3，and isoelectric points ranged from 4.95 to 9.59. Phylogenetic analysis showed that CmDof proteins were divided into nine phylogenetic subgroups. The expression patterns of CmDof genes in different tissues showed spatiotemporal expression specificity. Additionally，most CmDof genes could respond to PEG6000，salt and chilling stresses. CmDof3/CmDof5/CmDof10/CmDof17，CmDof3/CmDof5/CmDof10 and CmDof3/CmDof5/CmDof32 were responsive to PEG6000，salt and chilling stresses，respectively.

Key words: melon, Dof, gene family, phylogeny analysis, expression characteristic