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园艺学报 ›› 2019, Vol. 46 ›› Issue (11): 2099-2108.doi: 10.16420/j.issn.0513-353x.2019-0049

• 研究论文 • 上一篇    下一篇

桃PpSnRK1蛋白激酶对植株根系生长的影响

张淑辉1,王贵芳2,罗静静1,陈晓璐1,肖元松1,彭福田1,*   

  1. 1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018;2山东省果树研究所,山东泰安 271000
  • 出版日期:2019-11-25 发布日期:2019-11-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-30-2-02);山东省自然科学基金项目(ZR2017BC017);山东省“双一流”建设奖补资金项目(SYL2017YSTD10)

Effects of Peach PpSnRK1 Protein Kinase on Root Growth

ZHANG Shuhui1,WANG Guifang2,LUO Jingjing1,CHEN Xiaolu1,XIAO Yuansong1,and PENG Futian1,*   

  1. 1College of Horticultural Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China;2Shandong Institute of Pomology,Tai’an,Shandong 271000,China
  • Online:2019-11-25 Published:2019-11-25

摘要: 为探讨桃PpSnRK1蛋白激酶对植株根系生长的影响,以桃[Prunus persica(L.)Batsch]实生苗为试材,通过外源施加水杨酸(SnRK1促进剂)和海藻糖(SnRK1抑制剂)调节植株体内SnRK1活性,观测其对植株根系构型及活力的影响。从桃叶片克隆到PpSnRKα(Ppa004347m)目的基因,通过农杆菌介导遗传转化获得超表达PpSnRKα拟南芥植株,以T3代纯合株系为试材,观测PpSnRKα对拟南芥根系构型及根系中生长素合成和转运基因表达的影响。结果表明,无论是瞬时还是长期施加水杨酸,桃幼苗根系PpSnRK1α表达量与SnRK1酶活性都上调,且促进了根系生长,提高了根系活力,而施加海藻糖后抑制了根系生长和根系活力,当同时施加水杨酸 + 海藻糖(1︰1,体积比)后,SnRK1酶活性及PpSnRK1α表达量介于上述两个处理之间,且部分缓解了海藻糖对根系生长的抑制作用。通过观察超表达PpSnRKα拟南芥4-1、4-2、4-3纯合株系的根系可知,SnRK1可以增加根系的长度和密度,特别是增加了侧根的数量;通过RT-qPCR技术分析,超表达PpSnRKα拟南芥的3株纯合株系根系中,无论是生长素合成相关基因(AtTAA1、AtYUC2、AtYUC6),还是生长素运输相关基因(AtPIN1、AtPIN2、AtPIN3)表达量相比野生型均明显上调,且外施0.1 mg ? L-1 IAA的野生型株系与3株超表达PpSnRKα拟南芥纯合株系的根系表型相似,根系密度、长度及侧根数量增加,说明SnRK1对植株根系构型的影响与生长素信号途径密切相关,其可以正向调控生长素的合成与转运,进而正向调控根系生长,特别是侧根生长。

关键词: 苹果, 外源基因, 花粉, 育性, 绒毡层, 桃, SnRK1, 根系构型, 根系活力, 生长素

Abstract: To investigate the effect of peach PpSnRK1 protein kinase on plant root growth,the seedling of peach[Prunus persica(L.)Batsch] was used as test material,the activity of SnRK1 in plants was regulated by exogenous application of salicylic acid and trehalose,and the effects on root structure and activity were observed. From the leaves of peach to the target gene of PpSnRKα(Ppa004347m),the PpSnRK1α-overexpressed Arabidopsis plants was obtained through Agrobacterium- mediated genetic transformation,the T3 lines was obtained through screening to observe the influence on the root structure,and auxin synthesis and transporter gene expression of the plant. The results showed that the SnRK1 enzyme activity and PpSnRK1α gene expression in peach seedling roots were up-regulated when the SnRK1 promoter salicylic acid was applied instantaneously or for a long time,and the root growth and root activity were positively regulated. However,the application of SnRK1 inhibitor trehalose inhibited the root growth and root activity. When salicylic acid and trehalose 1︰1(V︰V)was applied together,SnRK1 enzyme activity and PpSnRK1α gene expression were between the two treatments,and the inhibition of trehalose on root growth was partially alleviated. By observing the root of the PpSnRK1α-overexpressed Arabidopsis plants 4-1,4-2,4-3 homozygous lines,it can be seen that SnRK1 can increase the length and density of the roots,especially the number of lateral roots. By RT-qPCR technical analysis,the PpSnRK1α-overexpressed Arabidopsis three homozygous strain root,whether Auxin synthesis related genes(AtTAA1,AtYUC2,AtYUC6),and Auxin transport related genes(AtPIN1,AtPIN2,AtPIN3)expression quantity compared with wild type were significantly higher,and the root phenotype of exogenous applied 0.1 mg ? L-1 IAA wild type strain and three PpSnRK1α-overexpressed Arabidopsis plants is similar,root density,length,and lateral root number increased. This indicated that SnRK1’s effect on plant root system configuration was closely related to the auxin signaling pathway,which could positively regulate the synthesis and transport of auxin,and then positively regulate root growth,especially lateral root growth.

Key words: apple, exogenous gene, pollen;viability, tapetum, peach, SnRK1, the root structure, root activity;auxin

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