关键词: 苹果, NAC转录因子, 酵母单杂交, 荧光素酶报告, 亚细胞定位, 黄酮醇

Abstract: In order to explore the mechanism of flavonol synthesis in red flesh apple，we analyzed the pathway-related genes using mature red flesh apples，and cloned a transcription factor gene MdNAC9. The correlation between MdNAC9 and flavonol accumulation was explored by Real-Time PCR，yeast one-hybrid and luciferase reporter assay. The results showed that the expression of MdNAC9 was positively correlated with MdFLS，a key gene in flavonol synthesis，in fruit and over-expressed callus （OENAC9）. Yeast one-hybrid showed that MdNAC9 could specifically bind to MdFLS promoter. Luciferase reporter assay proved that MdNAC9 could promote the expression of MdFLS. MdNAC9 may promote flavonol accumulation by activating MdFLS.

Key words: apple, NAC transcription factor, yeast one-hybrid, luciferase reporter assay, subcellular localization, flavonol