关键词: 苹果茎痘病毒, 内蒙古, RACE, 重组

Abstract: One Apple spot pitting virus（ASPV）isolate NM was collected from an infected‘Jinhong’apple in Hohhot，Inner Mongolia，China，and its complete genome sequence was determined by RT-PCR and RACE techniques. The Genbank accession No. is MK239268. The single-stranded positive RNA genome of ASPV-NM had 9286 nucleotides, excluding the poly A tail, and its shared sequence identities with the 17 published ASPV genomes in NCBI were from 70.6% to 79.2%. The phylogenetic trees based on complete genome，RNA-dependent RNA polymerase（RdRp）and Coat protein（CP）sequences showed ASPV-NM clustered into Group Ⅲ，Ⅲ andⅠ，respectively. One clear recombination event was identified in the isolate ASPV-NM. The ASPV-NM was sap-inoculated into Nicotiana occidentalis 37B，causing typical symptoms of yellow spots and chlorosis，and the infection was further confirmed by the transmission electron microscope.

Key words: Apple stem pitting virus, Inner Mongolia, RACE, recombination