关键词: 甘蓝, BoPMEI基因家族, 生物信息学, 表达分析

Abstract: In order to clarify the number of members，structures and distribution of BoPMEI family，and find out the relationship between BoPMEI family and self-incompatibility，transcriptome data and bioinformatics approaches were used to analyze the gene structure and identify the whole genome. The expression profile of BoPMEI family genes was analyzed，and it was verified by quantitative real-time PCR. The results showed that a total of 101 BoPMEI genes were identified and they were divided into 11 groups. The expansion of BoPMEI family genes distributed unevenly on 9 chromosomes were mainly caused by whole genome triplication and tandem duplication. The divergence time was determined to be 15.56 million years ago. Among the 101 genes，9 genes，such as BoPMEI56，BoPMEI54，BoPMEI32，BoPMEI29，BoPMEI78，BoPMEI30，BoPMEI57，BoPMEI58 and BoPMEI84，had significant expression differences. qRT-PCR analysis revealed that the mRNA expression patterns of BoPMEI was similar to the RNA-seq analysis. These results indicated that at least 9 of 101 gene members were involved in the regulation of self-pollination and cross-pollination of Brassica oleracea in different ways and may be related to the self-incompatibility of Brassica oleracea.

Key words: Brassica oleracea, BoPMEI family genes, bioinformatics, expression analysis