关键词: 草莓, FaWRKY31, 序列特征, 表达分析, 亚细胞定位

Abstract: To clarify the sequence characteristics，expression patterns and subcellular localization of FaWRKY31 in octoploid strawberry cultivar‘Benihoppe’，three CDS sequences with a length of 1 644 bp，one CDS sequence with a length of 1 669 bp and two promoter sequences about 2 000 bp were isolated by homology cloning strategy. Bioinformatics analysis indicated that the open reading frame of FaWRKY31-like with a length 1 669 bp was terminated prematurely because of a 25 bp sequence insertion，resulting the truncation of WRKY domain as well as C2H2 Zinc finger. Sequence analysis of promoter showed that there was a large divergence between FaWRKY31 promoter sequence and FvWRKY31. The similarity of those two promoter sequences was 70%，FaWRKY31 promoter existed some deletion and insertion of multiple fragments，which may lead to different inducing characteristics compared with FvWRKY31（F. vesca）. The gene expression level of FaWRKY31 evaluated by q-PCR showed that FaWRKY31 was detected in all analyzed tissues. The highest relative expression level was in roots，followed by stems and functional leaves and the lowest relative expression was in fruits. FaWRKY31 could be induced by red and blue light treatments，and be downregulated by blue and red light in strawberry fruits. FaWRKY31 can also significantly induced by low potassium，low phosphorus，ABA，drought，salt stress and low temperature in different degrees，and low temperature，ABA，drought had negative effects on FaWRKY31. Subcellular location of FaWRKY31 showed that FaWRKY31 was a nuclear locatized protein.

Key words: strawberry, FaWRKY31, sequence characteristics, expression patterns, nuclear location