谷胱甘肽提高月季切花失水胁迫耐性与GR活性的关系

园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 89-94.

谷胱甘肽提高月季切花失水胁迫耐性与GR活性的关系

王子华;金基石;高俊平

(中国农业大学观赏园艺与园林系, 北京100094)

收稿日期:2005-03-23 修回日期:2005-05-08 出版日期:2006-02-25 发布日期:2006-02-25

Effect of GSH Pretreatmen t on the Tolerance to Water Deficit Stress in Cut Rose ‘Samantha’and Related to Enhancement of GR Activity

Wang Zihua; Jin Jishi; Gao Junping

(Departm ent of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100094, China)

Received:2005-03-23 Revised:2005-05-08 Online:2006-02-25 Published:2006-02-25

摘要/Abstract

摘要： 本研究确定了切花月季‘Samantha’预处理采用的谷胱甘肽(GSH) 及其生物合成抑制剂丁胱亚磺酰胺〔L-buthione (S, R) sulfoximine, BSO〕适宜浓度, 探讨了GSH和BSO预处理对花朵水势、花瓣中MDA含量、GSH含量以及GR活性等的影响。结果表明: 与失水胁迫对照处理相比较, 2 mmol/L GSH预处理明显提高了月季切花瓶插期间花朵水势, 显著降低了花瓣中MDA含量, 有效提高了花瓣中GSH含量和GR活性。2 mmol/L BSO预处理获得了理想的反证结果。结果说明, GSH对切花月季‘Samantha’失水胁迫耐性的改善与花瓣中GR活性的提高相联系。

关键词: 月季, 切花, 失水胁迫, 谷胱甘肽, 谷胱甘肽还原酶

Abstract:

In this article, the op timum p retreated concentrations of GSH (glutathione) and its biosynthetical inhibitor, BSO〔L-buthione ( S, R) sulfoximine〕, were determined firstly according to their effects on vase quality of the flowers after 24 h water deficit stress in cut rose (Rosa hybrida) ‘Samantha’. Effects of 2 mmol /L GSH and 2 mmol/L BSO pretreatments for 12 h on water potential of flower bud, MDA (malondialdehyde ) and GSH contents, and GR (glutathione reductase) activity in petalswere investigated. The results showed that GSH pretreatment markedly increased water potential, but significantly decreased MDA content, GSH content and GR activity obviously increased during vase when compared with stress control flowers. The contrary results were obtained in 2 mmol/L BSO pretreated flowers. These results above indicate that the improvement of GSH on tolerance to water deficit stress is possibly be related to the enhancement of GR activity in petals of cut rose 'Samantha’.

Key words: Rose, Cut flower, Water deficit stress, GSH, Glutathione reductase

王子华;金基石;高俊平. 谷胱甘肽提高月季切花失水胁迫耐性与GR活性的关系[J]. 园艺学报, 2006, 33(1): 89-94.

Wang Zihua;Jin Jishi;Gao Junping. Effect of GSH Pretreatmen t on the Tolerance to Water Deficit Stress in Cut Rose ‘Samantha’and Related to Enhancement of GR Activity[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 89-94.

[1]	张爱玲, 涂红艳, 肖望, 钟晓晴, 陆秋婵, 成丽萍, 林晓萍, 麦钰玲. 白姜花二倍体与四倍体切花形态与显微结构变化观察[J]. 园艺学报, 2023, 50(2): 345-358.
[2]	欧阳丽莹, 黄艳飞, 陈君梅, 阳淑, 练华山. 微型月季新品种‘九儿’[J]. 园艺学报, 2022, 49(S2): 223-224.
[3]	练华山, 欧阳丽莹, 吴珊, 陈君梅, 舒彬. 微型月季新品种‘夜华’[J]. 园艺学报, 2022, 49(S2): 225-226.
[4]	陈君梅, 练华山, 黄艳飞, 禹婷, 蒋天仪. 微型月季新品种‘流星’[J]. 园艺学报, 2022, 49(S2): 227-228.
[5]	贾鑫, 曾臻, 陈月, 冯慧, 吕英民, 赵世伟. 月季‘月月粉’RcDREB2A的克隆与表达分析[J]. 园艺学报, 2022, 49(9): 1945-1956.
[6]	李茂福, 杨媛, 王华, 范又维, 孙佩, 金万梅. 月季中R2R3-MYB基因RhMYB113c调控花青素苷合成[J]. 园艺学报, 2022, 49(9): 1957-1966.
[7]	杨秀伟, 苏江硕, 张飞, 管志勇, 房伟民, 陈发棣. 切花小菊花序开放角度的量化评价与变异分析[J]. 园艺学报, 2022, 49(8): 1723-1734.
[8]	王妍, 孙政, 冯珊, 袁心怡, 仲林林, 曾云流, 傅小鹏, 程运江, 包满珠, 张帆. 香石竹DcERF-1转录因子对切花衰老的负调控作用[J]. 园艺学报, 2022, 49(6): 1313-1326.
[9]	李丽仙, 王烁, 陈莹, 邬滢涛, 王雅倩, 房月, 陈学森, 田长平, 冯守千. 甜樱桃PavMYB10.1促进PavRiant表达和花青苷积累[J]. 园艺学报, 2022, 49(5): 1023-1030.
[10]	赵立民, 李嘉伟, 张飞, 苏江硕, 房伟民, 王海滨, 蒋甲福, 陈素梅, 陈发棣, 管志勇. 基于表型数据构建切花小菊核心种质[J]. 园艺学报, 2022, 49(10): 2273-2284.
[11]	李茂福, 杨媛, 王华, 范又维, 孙佩, 金万梅. 月季自交不亲和性S-RNase的鉴定与分析[J]. 园艺学报, 2022, 49(1): 157-165.
[12]	王晶晶, 郁琳洁, 陆小雨, 柳斌, 史丹, 陈竹, 唐菲, . 鸢尾新品种‘金丰双星’[J]. 园艺学报, 2021, 48(S2): 2899-2870.
[13]	张文心, 曹雪莹, 周锦, 周世明, 张别鱼, 房林, 李琳, 吴坤林, 曾宋君, . 朱顶红新品种‘圣茵3号’[J]. 园艺学报, 2021, 48(S2): 2909-2910.
[14]	胡秀, 钟玉成, 谭嘉川. 姜花新品种‘寒月’[J]. 园艺学报, 2021, 48(S2): 2925-2926.
[15]	王依, 王凯轩, 胡思源, 周爽, 史国安. 乙烯代谢和能量状态对‘巴茨拉’牡丹切花瓶插品质的作用研究[J]. 园艺学报, 2021, 48(6): 1135-1149.