关键词: 菠菜, 全基因组, 关联分析, 雌雄同株

Abstract:

Spinach is a typical dioecious plant，but some varieties produce monoecious plant. In order to locate the genes controlling the monoecious traits of spinach，the sexual genotypes of 82 spinach inbred lines were investigated and analyzed. Each sample isolated from these accessions was re-sequenced with 10-fold sequencing depth，and a total of 3 420 496 SNPs were obtained. After filtration，1 621 970 high-quality polymorphic SNPs were acquired. The genome-wide association study（GWAS）of monoecious was performed by using 1 621 970 high-quality SNPs. The compress mixed linear model（cMLM）was used for genome-wide association analysis（GWAS）of monoecious traits in spinach using Genome Association and Prediction Integrated Tool（GAPIT）software. And a strong correlation region was detected on the chromosome 4. Based on the size of linkage disequilibrium block（31.2 kb），Xm gene was located in 64.6 kb region. Three genes，Spo24600，Spo24601 and Spo24602，were located in this region. In this study，KASP marker was developed in the correlation region，which can be used for the identification of monoecious spinach.

Key words: spinach, GWAS, monoecism