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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1279-1289.doi: 10.16420/j.issn.0513-353x.2018-0448

• 研究论文 • 上一篇    下一篇

杧果成年树在增强UV-B辐射处理下的损伤与抗氧化响应

周开兵*,李世军,袁孟玲,岳 堃   

  1. 海南大学热带农林学院,海口 570228
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    国家自然科学基金项目(31460498)

Injuries and the Responses on Antioxidation of Adult Mango Trees Under the Treatments of Enhanced UV-B Radiation

ZHOU Kaibing*,LI Shijun,YUAN Mengling,and YUE Kun   

  1. College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 以10年生‘金煌’杧果为试验材料,以自然光照为对照,设置了人工模拟增强 UV-B 辐射24、48、72、96、120 kJ ? m-2 ? d-1处理,观测处理后单株产量、品质和与叶片生理损伤、抗氧化保护机制等有关的生理生化指标的动态变化。结果表明,增强UV-B辐射剂量大于48 kJ ? m-2 ? d-1时,辐射强度越强,杧果减产、果实变小和风味营养品质变劣越严重,叶片叶绿素含量和净光合速率下降加重,MDA含量和相对电导率升高愈趋强烈;SOD活性表现为72 kJ ? m-2 ? d-1以下处理高于对照和96 kJ ? m-2 ? d-1以上处理低于对照的趋势; 72 kJ ? m-2 ? d-1以下处理的POD活性高于对照,120 kJ ? m-2 ? d-1处理的POD活性低于对照,后期96 kJ ? m-2 ? d-1处理时,显著低于对照;CAT活性表现为所有处理均高于对照。所有处理均能促进还原型GSH、类黄酮类化合物和多酚类化合物含量升高,同时能使维生素C含量降低。可见,48 kJ ? m-2 ? d-1辐射处理引起树体损伤、减产和果实品质变劣,且呈现出剂量效应,树体损伤还呈现积累效应;72 kJ ? m-2 ? d-1处理通过诱导叶片抗氧化酶系活性增强,具备抗氧化和吸收UV-B辐射双重功能的成分积累增加,维生素C消耗增多等,尽可能提高清除活性氧自由基的能力和耗散增强UV-B辐射,从而尽量减轻树体损伤;96 kJ ? m-2 ? d-1以上的处理则抑制了SOD和POD活性,后期抗氧化成分和吸收UV-B辐射的成分积累,导致活性氧自由基积累增加和UV-B胁迫加强,进而引起更严重的损伤。

关键词: 杧果, 增强UV-B辐射, 损伤, 抗氧化

Abstract: In order to research the effects of the enhanced UV-B radiation on the injuries and the antioxidation responses of adult mango trees,the adult‘Jinhuang’mango trees in the field were treated with the enhanced UV-B radiation from 24 to 120 kJ ? m-2 ? d-1 in gradient of 24 kJ ? m-2 ? d-1,and those in the natural sunlight were used as the control. The yield of single tree,the qualities of fruits and the tendencies of the physiological and biochemical indicators related to the injuries and the antioxidation of leaves were detected during the period of the experimental treatments. With the increases of the intensities of the enhanced UV-B radiation over 48 kJ ? m-2 ? d-1,the yield of single tree,the size of fruit and the flavor and nutrient qualities decreased more and more. With the increase of the intensities of the enhanced UV-B radiation,the content of chlorophyll and the level of Pn decreased more and more,and the content of MDA and the relative conductivity increased more and more. The activities of SOD of the control were lower than those of the treatments below 72 kJ ? m-2 ? d-1 and higher than those of the treatments over 96 kJ ? m-2 ? d-1. The activities of POD of the control were lower than those of the treatments below 72 kJ ? m-2 ? d-1 and higher than those of the treatment 120 kJ ? m-2 ? d-1 all the time and the treatment 96 kJ ? m-2 ? d-1 during the late period. The activities of CAT of the control were lower than those of all the treatments all the time. All the treatments decreased the content of vitamin C and increased the contents of the components such as reduced GSH,flavonoid and polyphenol. In sum,the enhanced UV-B radiation over 48 kJ ? m-2 ? d-1 injured the trees,reduced the yield and made the fruits qualities getting worse with dosage effects,and the damage in trees appeared accumulation effects. The treatments below 72 kJ ? m-2 ? d-1 intensified the activities of antioxidative enzymes,and increased the substances accumulations of antioxidant and absorption of UV-B radiation,and consumed more vitamin C,which reinforced free radicals scavenging abilities and dissipation of enhanced UV-B radiation as far as possible,then minimized UV-B radiation damage. The treatments over 96 kJ ? m-2 ? d-1 inhibited the activities of SOD and POD and reduced the substances accumulations of antioxidant and absorption of UV-B radiation during the experiment,which resulted in cumulating more free radicals and strengthening the stress of the enhanced UV-B radiation,then led to more serious injury to plants.

Key words: mango, enhanced UV-B radiation, injury, antioxidation

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