关键词: 葡萄, 非生物胁迫, 基因家族, 生物信息学分析, 基因表达芯片, qRT-PCR

Abstract: The characteristics and functions of grape Trihelix transcription factor family genes were analyzed and predicted by bioinformatics，providing a theoretical basis for the mining and utilization of grape stress resistant gene. In this study，Vitis vinifera was used as the material. Bioinformatics was used to analyze the physicochemical properties，chromosomal localization，secondary structure prediction，subcellular localization，gene structure analysis，motif explores and gene chip expression of grape Trihelix transcription factor family. The qRT-PCR was used to analyze the expression of this family under 400 mmol ? L-1 NaCl，100 mmol ? L-1 ABA and 10% PEG stress. The results showed that there were 27 members of the transcription factor family in the grape genome，and distributed on 11 chromosomes，including 10 members concentrated on chromosomes 14 and 17. The amino acid size is mainly between 175–880 aa，most is hydrophobic. The subcellular localization analysis indicated that this gene family mainly existed in the nucleus. Domain analysis displayed that the conserved domain structure of the same subfamily was highly similar. Gene chip expression results clearly illuminated that VvTrihelix6 played up-regulated trend after 24 h PEG treatment compared with the control under abiotic stress，and a down-regulated trend after 4 h and 24 h PEG treatment contrast with the control. Also，after ABA treatment for 3 d，the VvTrihelix6 was down-regulated in fruits，but the change was not obvious after 10 d. The transcription factor VvTrihelix19 showed a down regulation trend after 24 h treatment with PEG，salt and cold compare with control，and showed significant down-regulation at 3 d and 10 d under ABA treatment. qRT-PCR showed that all genes in the Trihelix transcription factor family responded to stress. However，the response degree of members of the gene family was different under different stress conditions. The expression of transcription factor VvTrihelix6 in 400 mmol ? L-1 NaCl and 100 mmol ? L-1 ABA treatment was significantly higher than the control group，15 times and 8 times of the control，respectively. VvTrihelix19 was significantly up-regulated after 10% PEG stress treatment.

Key words: grape, abiotic stress, gene family, bioinformatics analysis, gene expression microarray, qRT-PCR