关键词: 杜鹃, 盐胁迫, cDNA-SCoT, 差异表达基因

Abstract: Soil salinization is one of the main factors that limit the distribution and landscape application of Rhododendron. To explore the molecular mechanism of Rhododendron resistance to salt stress，R.‘Jiangnan Chunzao’and R. obtusu‘Yanzhi Mi’seedlings were used as the materials to analyse different expressed genes under salt stress by the method of cDNA-SCoT. The results showed that 40 primers could amplify more than 90 bands，most of which were between 100-1800 bp in length. Thirty two bands with obvious differences were selected for recovery. Fifteen high-quality EST sequences were obtained by sequencing after the transformation. Bioinformatics analysis showed that the differential genes in Rhododendron seedlings exposed to salt stress mainly coded catalase，LRR receptor-like serine / threonine protein kinase At1g63430 Structure X1，Shc-related protein，photosystem Ⅱ protein D1，PTS fructose transporter subunit ⅡABC etc，and involved in cell structure，defense response，metabolism，energy metabolism，signal transduction，transport and transcription etc.

Key words: Rhododendron, salt stress, cDNA-SCoT, different expressed genes