关键词: 金花茶, 杂交品种, 类黄酮, 花色, 超高效液相色谱–四极杆–飞行时间质谱

Abstract: The flower colors，flavonoid components and contents in dark yellow Camellia nitidissima，white C. japonica and their pale yellow hybrid cultivars were studied. The flower colors were measured by CIE L*a*b* scale，and flavonoids were qualitatively and quantitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry. The relationship between flower colors and flavonoid components was explored by analyzed by multiple liner regression analyses. Ten flavonoids were detected in C. nitidissima，white C. japonica and their three hybrid cultivars. The flavonoids including pelargonium-3-O-glucoside（Pg3G），luteolin-7-O-rutinoside（Lu7R），eriodictyol（Er）and genistin（Gin）were identified in C. nitidissima for the first time. Pg3G，Er and Gin were not determined in white C. japonica‘Yinbai Chalis’and‘Baifeng’，and the contents of Lu7R were extremely low. The main components of C. nitidissima were quercetin-3-O-glucoside（Qu3G），quercetin-3-O-rutinoside（Qu3R），quercetin-7-O-glucoside（Qu7G），kaempferol-3-O-glucoside（Km3G），Pg3G and Lu7R. The contents of luteolin（Lu），kaempferol（Km）and Er in hybrid cultivars surpassed their parents，and those of the rest and total flavonoids were between their parents. Qu3G and Qu3R were the important components which decided the yellow color of C. nitidissima and its hybrid cultivars，and the increase of their contents caused the flower color to remarkably turn yellow. The accumulation of Qu3G significantly enhanced the brilliance of flower color，and Pg3G affected the color tone.

Key words: Camellia nitidissima, hybrid cultivar, flavonoids；flower color, ultra performance liquid chromatography quadrupole-time-of-flight mass spectrometry