关键词: 梅, 组织培养, 离体快繁

Abstract: The op timum in vitro shoot section exp lants growing medium for Prunus m um e cultivar Tiegu
Hong was the modified Quoirin and Lepoivre medium supp lemented with Quoirin and Lepoivre medium large
elements, Perezmedium trace elements, Murashige and Skoogmedium (MS) organic compounds, and double
MS Fe salt. Thismodifiedmedium was used to resolve a serious of problems faced in Tiegu Hong tissue culture
such as seedling chlorosis and shoot tip death. The op timum p roliferation medium for Tiegu Hongwas the im
p roved Quoirin and Lepoivre medium supp lemented with 1.0 mg·L ^-1 6-BA, 0.05 mg·L ^-1 NAA and 4 mg·L^-1 AgNO_3. Attention should be paid to the app lication ofAgNO₃ because of long period of use may lead to
the p lantletsmalformation. The op timum rooting medium for Tiegu Hong was the imp roved 1 /2 Quoirin and
Lepoivre medium supp lemented with 0.3 mg·L ^-1 NAA and 0.1 mg·L ^-1 IBA.

Key words: Prunus mume, Tissue culture, Fast in vitro micro-propagation