关键词: 百合, 花青素苷, 呈色类型, R2R3-MYBs

Abstract: Anthocyanin accumulation in different parts of tepal developed various pigmentation patterns，but the expression patterns of R2R3-MYBs regulating various anthocyanin pigmentation patterns in Lilium spp. are not well understood. In this study，expression patterns of LhMYB12，LhMYB6 and LrMYB15 genes regulating various anthocyanin pigmentation patterns were performed in seven different pigmentation patterns of materials[Asiatic hybrids，LA（Lilium longiflorum × Asiatic hybrids）and wild species]. LhMYB12 and LhMYB6，cloned full-length cDNA and analyzed gene sequences，were considered to be regulated gene related to anthocyanin pigmentation patterns. The study showed LhMYB12 was expressed in linear spots at edge of tepals and splatter spots in Asiatic hybrids. But LhMYB12 and LhMYB6 were expressed in linear spots at edge of tepals and splatter spots in LA hybrids. And only LhMYB12 was expressed in anthocyanin pigmentation patterns of wild species（L. rosthornii，L. henryi and L. martagon）. However，anthocyanin pigmentation patterns of these parameters were not observed in LrMYB15. The full-length of LhMYB12 and LhMYB6 was 763 and 797 bp containing open reading frame of 744 and 705 bp，which encoded 247 and 234 amino acids respectively. They were homologous sequence with published LhMYB12，LhSorMYB12 and LhMYB6，sharing 78% to 88% similarity with them. The results indicated that LhMYB12 played an important role in the regulation of anthocyanin pigmentation patterns in hybrids and wild species widely. Moreover，LhMYB12 and LhMYB6 regulated anthocyanin in LA hybrids. This study revealed that the expression of R2R3-MYBs was involved in regulating various anthocyanin pigmentation patterns in different species.

Key words: Lilium, anthocyanin, pigmentation pattern, R2R3-MYBs