关键词: 苹果, 花青苷, MdMYB111, 抑制序列, 功能鉴定, 甜瓜, 品种

Abstract: The fruit of‘Hongxin 16’apple，a progeny of Malus sieversii f. neidzwetzkyana hybrids，was used as the test material. MdMYB111 was cloned and its recombinant protein was induced and expressed in prokaryotic analysis. The bioinformatic analysis of this gene was conducted，its expression level was determined in the fruits of the varieties of‘Hongxin 16’and‘Hongcui 1’and its function was identified by transgene. The results showed that the open reading frame of MdMYB111 was 720 bp，encoding 239 amino acids，and its predicted protein size was 26.93 kD. Phylogenetic tree analysis showed that MdMYB111 and MdMYB16 were in the same clade and there was an EAR inhibitory sequence at the C-terminus of the protein. The ripened fruit of‘Hongxin 16’ is complete red and darker in color. The fruit of‘Hongcui 1’has a light red color and a small red range. On the‘Hongxin 16’，both the content of anthocyanin in fruits mature stage and MdANS，MdUFGT，MdMYB9，MdMYB10，MdMYB11 expression levels were higher than that of‘Hongcui 1’，but the expression levels of MdMYB16 and MdMYB111 were lower than‘Hongcui 1’. Overexpression of MdMYB111 in red apple callus can inhibit the expression of MdANS and MdUFGT and reduce the content of anthocyanin. In summary，MdMYB111 may be involved in the anthocyanin synthesis pathway and inhibit its synthesis.

Key words: apple, anthocyanin, MdMYB111, inhibitory sequence, functional identification, melon, cultivar