https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 1041-1046.

• 研究简报 • 上一篇    下一篇

菊花品种资源遗传多样性的AFLP分析

韩 洁1; 胡 楠2; 李玉阁1; 尚富德1*
  

  1. (1河南大学生命科学学院, 农业生物技术研究所, 河南开封475001; 2 南阳理工学院, 河南南阳473000)
  • 收稿日期:2007-05-08 修回日期:2007-07-25 出版日期:2007-08-25 发布日期:2007-08-25

Genetic Diversity of Chrysanthemum Cultivars Revealed by AFLP Analysis

HAN Jie1; HU Nan2; LI Yu-ge1; SHANG Fu-de1*
  

  1. (1 College of Life Sciences and Institute of Agricultural Biotechology, Henan University, Kaifeng, Henan 475001, China;2Nanyang Institute of Technology, Nanyang, Henan 473000, China)
  • Received:2007-05-08 Revised:2007-07-25 Online:2007-08-25 Published:2007-08-25

摘要: 以AFLP—银染分子标记技术, 对45个菊花品种进行了遗传多样性和亲缘关系分析。选用10
个多态性高、分辨力强的E + 3 /M + 3引物组合分别对供试材料的基因组DNA进行扩增, 共获得486条清
晰可辨的条带, 其中多态性带451条, 平均每个引物组合可检测出4511个多态性位点, 多态性位点百分率
高达92.80% , 这表明供试品种资源在DNA水平上酶切位点的分布存在广泛的变异。应用DPS软件计算供
试品种遗传距离界于0.36000~0.86237之间, 平均为0.611185; UPGMA分析将45份资源分成6个类群,
从相异性系数分析了各品种资源间的亲缘关系。

关键词: 菊花, AFLP, 遗传多样性, 亲缘关系

Abstract: The genetic diversity and relationship of 45 chrysanthemum cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol. AFLP fingerprinting of 45 chrysanthemum cultivars with ten pairs of E+3/M+3 primers revealed a total number of 486 unambiguous bands, of which 451 were polymorphic and 45.1 polymorphic bands per pair of primer were produced on average. The polymorphism frequency was 92.80%. This result showed the abundant diversities of enzyme digestion sites
among used chrysanthemum cultivars. As analyzed by DPSv3.01, the Neips genetic distance of 45 chrysanthe
mum cultivars ranged from 0.36000 to 0.86237, and the average distance was 0.611185. These chrysanthe
mum cultivars were divided into six group s by UPGMA ( unweighted pair group method with arithmetic aver
age) based on Neips genetic distances. The genetic relationship of 45 chrysanthemum cultivars was analyzed
using the different coefficient.

Key words: Chrysanthemum, AFLP, Genetic diversity, Relationship