关键词: 小西葫芦黄花叶病毒, 分离物, 全基因组序列, 系统发育分析, 重组分析, 黄瓜, 果实发育, 基因, 农艺性状, 分子机制

Abstract: Zucchini yellow mosaic virus（ZYMV）is a major virus infecting cucurbit crops. ZYMV was detected in leaf samples of loofah and pumpkin displaying symptoms of yellowing and mosaic collected from Tai’an，Shandong Province. Their complete genome sequences were determined using RT-PCR and 5′ RACE，the resulting complete genomes were named as CN:Cm:17 and CN:Lc:17，respectively. Sequencing results showed that the complete genome excluding poly(A) tail of CN:Cm:17 and CN:Lc:17 was 9 593 and 9 591 nucleotides（nt），respectively，encoding a polyprotein of 3 080 amino acids. Amino acid sequence identity between CN:Cm:17 and CN:Lc:17 was 96.7%. Isolate CN:Cm:17 shared the highest sequence identity with KR:RDA:07 at amino acid level，CN:Lc:17 showed the highest sequence identity with CN:CJLX30535:14 and CN:spider131932:13. Recombinant analysis revealed that CN:Lc:17 was recombinant of isolates of KR:KR-PA:05 and CN:Cm:17. Phylogenetic analysis based on polyprotein- encoding sequences suggested a high correlation between phylo-groups and their geographical origins. All Chinese isolates were grouped in A-Ⅴ and A-Ⅵ，respectively.

Key words: Zucchini yellow mosaic virus, isolates, complete genomic sequence, phylogenetic analysis, recombination analysis, cucumber, fruit development, gene, agronomic traits, molecular mechanism