关键词: 月季, 赤霉素, 生长发育, 辣椒, 辣椒脉斑驳病毒, 抗病育种

Abstract: In this study，different concentrations of gibberellin（GA3）were applied to the branch of the rose，‘Corolla’，and the water spraying was used as the control. The initial flower date，the date of flower opening，and the date of flower withering were recorded and the length of flower branches，flower stalks，plant heights and the ratio of leaf longitudinal and horizontal were measured at full-bloom stage，in order to reveal the effects of GA3 on the growth and development of rose‘Carola’. When the treatment concentration of GA3 was 300 mg · L-1，the average length of the rose was the longest and the aspect ratio of the leaf was the largest；the treatment with the concentration of 300 and 400 mg · L-1 had the longest average length of the flower stalks；the concentration 500 mg · L-1 had the highest average plant height. Under the spraying condition of GA3 300 mg · L-1，RT-PCR results demonstrated that the application of GA3 up-regulated the expression of RhGA2ox，RhGA20ox，RhGA3ox，and RhGID1 genes，which might participate in the regulation of GAs metabolism and hormone signaling. Furthermore，the changes might indirectly induce the expression of genes related to auxin synthesis，leading to cell elongation and cell volume increase，which in turn promoted the increase of the plant height and the leaf length in rose plants. The investigation of related flowering-related genes，RhAP1，RhKSN and RhSOC1 showed that the expression pattern of RhAP1，RhFT and RhSOC1 gene expression levels were first to increase and then decrease after the application of GA3，and changed greatly，compared with the control. The high expression of the flowering gene，RhFT may be one of the causes of the earlier date for flowering due to GA3 treatment；in addition，the high expression of RhKSN may be one of the reasons for the longer period of flowering.

Key words: Rosa hybrida, gibberellin, growth and development, pepper, chilli veinal mottle virus, resistant breeding