关键词: 甘蓝, NLR家族基因, 抗病, 表达分析, 辣椒, 起源, 栽培种, 中国, 传播, 产业功能

Abstract:

In this study，based on the reference genome we identified 87 NLR family genes in Brassica oleracea and performed evolution，comparative，and expression analysis. Gene structures and motif composition analysis indicated that the 87 NLR genes were assigned to five subclasses：NB-LRR（34），CC-NB-LRR（6），TIR-NB-LRR（45），RPW8-NB-LRR（1）and CC-TIR-NB-LRR（1）. Chromosome location analysis revealed that the NLR genes were unevenly distributed in single or in clusters on nine chromosomes. Evolution analysis showed that 35 NLR genes were homologous with Chinese cabbage，while the other 52 genes were specific to cabbage. Expression profiling of NLR genes identified several differential expression genes related to resistance to Fusarium wilt，black rot，clubroot，downy mildew and powdery mildew in Brassica oleracea，and results showed that expression of NLR genes participating in resistance response to various diseases exhibited significant difference，which may indicate the specific recognition of NLR genes for diverse pathogens.

Key words: Brassica oleracea, NLR family gene, resistance, expression profiling, Capsicum spp., origin, cultivated species, China, spread, industrial function